SUMMARY
The MDR/XDR-TB Colour Test is a simple, safe, low-cost, appropriate-technology test for diagnosing TB and concurrently determining antibiotic-susceptibility testing. This test helps ensure that patients in resource-constrained settings receive the best treatment for their TB disease. The MDR/XDR-TB Colour Test was invented and developed by the IFHAD research group in Peru and has since been evaluated in multiple countries – please see the publications below.
PROTOCOL
The current MDR/XDR-TB Colour Test protocol may be downloaded from this link: https://www.dropbox.com/sh/hircpcbcs2cv6c3/AADeTbxlyH6pX8cF1TLMR1LRa?dl=0
This file includes links to previous versions of the protocol.
Further information is available from carlton.evans@ifhad.org
PUBLICATIONS
B Herrera, R H Gilman, L Grandjean, L Martin, J Alvarado, W Quino, T Valencia, G Sandhu, R Montoya, J Alva, J Franco, M Haro, R Sosa, E Valera,B Valiente, M Rivero, S Carrera, A R Escombe, A Curatola, C A Evans.
Optimisation of TB field testing: In transit sputum decontamination & culture on colorimetric selective media for TB diagnosis & drug-susceptibility testing.
Presentation at the Medecins Sans Frontieres (MSF) symposium on TB field Diagnostics ‘Dying for a test’. 7/11/2007 Capetown. http://www.msfaccess.org/fileadmin/user_upload/diseases/tuberculosis/DX_Abstacts_CT.pdf
S Shell, E Ramos, B Herrera, RH Gilman, W Quino, J Alvarado, T Valencia, G Sandhu, M Rivero, S Carrera, R Montoya, J Alva, J Franco, M Haro, R Sosa, E Valera, B Valiente, A Curatola, AR Escombe, D Moore, P Sheen, C Evans. Enhancing TB culture diagnosis with the colorimetric indicator STC American Society of Tropical Medicine & Hygiene conference 4/11/2007 Philadelphia.
OC Boney, K Zevallos1, RH Gilman, L Caviedes, T Valencia, CO Vidal, AR Escombe, CA Evans.
Evaluation of MODS vs. clinical assessment, sputum microscopy, culture and PCR for diagnosing patients with TB.
Presentation at the Medecins Sans Frontieres (MSF) symposium on TB field Diagnostics ‘Dying for a test’. 7/11/2007 Capetown. http://www.msfaccess.org/fileadmin/user_upload/diseases/tuberculosis/DX_Abstacts_CT.pdf
B Herrera, E Ramos, R Gilman, L Grandjean, L Martin, J Alvarado, W Quino, G Sandhu, J Alva, R Sosa, S Carrera, D Coleman, D Mitchison, C Evans.
A simple colour test for diagnosing MDRTB & XDRTB under field conditions.
CDC symposium, World Conference of the International Union Against TB & Lung Disease Paris, 20/10/2008.
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B Herrera, E Ramos , R Gilman, L Grandjean, L Martin, J Alvarado, T Valencia, W Quino, G Sandhu, J Alva, R Sosa, S Carrera, D Coleman, D Mitchison, C Evans.
Simplifying culture-based TB/MDRTB testing for Peripherals laboratories.
FIND Forum on Recent Advances in TB Diagnostics, Paris 17/10/2008. Abstract published in conference proceedings.
MA Tovar, T Valencia, RH Gilman, L Caviedes, E Ramos, J Alvarado, W Quino, B Herrera, CA Evans.
Validation of the MODS technique for drug-susceptibility testing during TB therapy.
American J Tropical Medicine & Hygiene 2009: 81(5); 212: page 212. Abstract#1317, Presentation Number: 734 Session 125 http://www.ajtmh.org/content/81/5_Suppl_1/201.full.pdf
MA Tovar, T Valencia, J Alvarado, C Rojas, L Caviedes, S Schumacher, R Gilman, C Evans
Rapid direct MDRTB testing better predicts clinical outcome than conventional delayed testing
International Journal of TB and Lung Disease 2010:14(11);S73.-74. http://www.theunion.org/what-we-do/journals/ijtld/body/ABSTRACT_BOOK_2010_Web.pdf
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SG Schumacher, T Valencia, R Montoya, M Tovar, A Gavino, S Carrera, RH Gilman, C Evans
Operational effectiveness of TB culture and drug-susceptibility testing in a high-prevalence setting.
International Journal of Tuberculosis and Lung Disease 2010:14(11);S72.http://www.theunion.org/what-we-do/journals/ijtld/body/ABSTRACT_BOOK_2010_Web.pdf
MA Tovar, C Osorio, E Ramos, M Llacza, B Herrera, SG Schumacher, RH Gilman, C EvansA simple colour test for diagnosing MDRTB & XDRTB under field conditions International Journal of TB and Lung Disease 2010:14(11);S73. http://www.theunion.org/what-we-do/journals/ijtld/body/ABSTRACT_BOOK_2010_Web.pdf
MA Tovar, SG Schumacher, C Osorio, E Ramos, M Llacza, T Valencia, B Herrera, CA EvansComparison of TB diagnosis with the MDR/XDRTB Colour Test and MODS International Journal of TB & Lung Disease 2011:15(11);S274. http://www.worldlunghealth.org/confLille/images/stories/AbstractBook2011_Web.pdf
Rocha C, Tilley H, Ching M, Ramos E, Tovar MT, Rivera E, Cortegana L, Evans CA.
Field assessment of TB culture techniques in a resource-poor jungle setting.
International Journal of TB & Lung Disease 2012:16(12);S430.http://www.worldlunghealth.org/conf2012/images/banners/RANDOMS/ABSTRACT_BOOK_2012_WEB.pdf
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K Toit, S Mitchell, Y Balabanova, CA Evans, T Kummik, V Nikolayevskyy, F Drobniewski
The Colour Test for drug susceptibility testing of TB strains by culture on thin-layer agar
International Journal of TB & Lung Disease 2012 Aug;16(8):1113-8. doi: 10.5588/ijtld.11.0609. http://www.ncbi.nlm.nih.gov/pubmed/22762424
ES Ramos, CE Osorio, TR Valencia, MF Llacza, MA Tovar, R Montoya, T Wingfield, CA Evans
The specificity of the MDR/XDR-TB Colour Test for differentiating Mycobacterium TB from atypical mycobacteria
http://www.bc.lung.ca/association_and_services/documents/2013TheUnion-NARPreliminaryProgram-FINALPROGRAM.pdf page 71
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J Wendell, B Hanson Herrera, E Ramos, MA Tovar, S Schumacher, T Valencia, D Coleman, CA Evans.
The MDR/XDR-TB Colour Test simultaneous culture and drug susceptibility results predict TB outcomes.
International Journal of TB & Lung Disease 2013: 17(12);S271. http://www.worldlunghealth.org/conf2013/images/1_Paris2013/Forms/ABSTRACT_BOOK_2013_Web.pdf
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ES Ramos, D Coleman, CE Osorio, TR Valencia, MF Llacza, MA Tovar, T Wingfield, CA Evans
Carbendazim reduces contamination of TB cultures in the thin layer agar MDR/XDR-TB Colour Test
International Journal of TB & Lung Disease 2013:17(12);S272. http://www.worldlunghealth.org/conf2013/images/1_Paris2013/Forms/ABSTRACT_BOOK_2013_Web.pdf
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Rocha C, Tilley DH., Ching M., Ramos E., Tovar MA., Rivera E., Cortegana L., Evans CA.
Comparison of time to MDR-TB diagnosis with the MDR/XDR-TB Colour Test & current algorithm in a resource-poor jungle setting in Peru
International Journal of TB & Lung Disease 2013:17(12);S472. http://www.worldlunghealth.org/conf2013/images/1_Paris2013/Forms/ABSTRACT_BOOK_2013_Web.pdf
Eric Steve Ramos, Carmen Osorio, Teresa Valencia, Manuel Llacza, Moises Ching, Marco Tovar, Alexander Sloutsky, Carlton A Evans
High TB diagnostic yield from culturing salivary sputum samples
International Journal of TB & Lung Disease 2014:18(11);S285 http://www.theunion.org/what-we-do/journals/ijtld/body/Abstract_Book_2014-Web.pdf
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Marco A Tovar, Teresa Valencia, Silvia Carrera, Eric Ramos, Tom Wingfield, Rosario Montoya, Maribel Rivero, Carlton A Evans
Undiagnosed tuberculosis cases among previously TB-affected families: results of a prevalence survey in Peru
International Journal of TB & Lung Disease 2014:18(11);S221 http://www.theunion.org/what-we-do/journals/ijtld/body/Abstract_Book_2014-Web.pdf
Marco A. Tovar, Eric Ramos, Teresa Valencia, Sumona Datta, Rosario Montoya, Adriana Valencia, Tom Wingfield, Carlton A. Evans
Comparison of the MDR/XDRTB Colour Test, Xpert MTB/Rif and MODS assays to diagnose rifampicin resistant TB
International Journal of TB & Lung Disease 2015:19(12);S368 http://www.theunion.org/what-we-do/journals/ijtld/body/Abstract_Book_2015-Web.pdf
Marco A. Tovar, Tom Wingfield, Matthew J. Saunders, Rosario Montoya, Eric Ramos, Teresa Valencia, Sumona Datta, Carlton A. Evans
Risk factors associated with undetected pulmonary TB in a prevalence survey, Callao, Perú
International Journal of TB & Lung Disease 2016:20(11);S255
http://www.theunion.org/what-we-do/journals/ijtld/body/Abstract_Book_2016-Web.pdf
Sumona Datta, Marco A Tovar, Teresa R Valencia, Eric S Ramos, Rosario Montoya, James Lewis, Carlton A. Evans
Sputum collection factors affecting diagnostic yield
International Journal of TB & Lung Disease 2017:21(11); S188 http://www.abstractserver.com/TheUnion2017/TheUnion2017_Abstracts_Web.pdf
Annie Zhang, Enoch Jumbe, Robert Krysiak, Sabeen Sidiki, Holden V. Kelley, Elly K. Chemey, Chancy Kamba, Victor Mwapasa, Juan I. García, Alison Norris, Xueliang J. Pan, Carlton Evans, Shu-Hua Wang, Jesse J. Kwiek, Jordi B. Torrelles
Low-cost diagnostic test for susceptible and drug-resistant tuberculosis in rural Malawi
African Journal of Laboratory Medicine 2018 Vol 7(1); a690 DOI:
Open access https://doi.org/10.4102/ajlm.v7i1.690
Sumona Datta, Marco A Tovar, Thomas Wingfield, Rosa Patrocinio, Rosario Montoya, Teresa Valencia, Robert H Gilman,Carlton A Evans.
Monitoring TB treatment response with conventional laboratory tests during the first 14-days of therapy has no clinical value.
European Conference clinical infectious diseases and microbiology, in press. Poster presentation, 24th April 2018.
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S Datta, D Huff, L Quevedo Cruz, J Quispe Cotache, R Montoya, MA Tovar, M Saunders, JJ Lewis, RH Gilman, CA Evans. A quantitative assessment of general wellbeing in TB-affected people
Oral abstract session OA20-328-27, Saturday, 27th October.
International Journal of TB & Lung Disease 2018:22(11); S434. https://www.abstractserver.com/TheUnion2018/TheUnion2018_Abstracts_Web.pdf
A. Shibabaw, B. Gelaw, H. Kelley, J. Balada-Llasat, C. Evans, S. Wang, J. Torrelles, B. Tessema.
Accuracy of the color plate micro-colony detection for the diagnosis of Mycobacterium TB complex in NW Ethiopia.
Tuberculosis. 2019; 114:54-60.
Biruk Mekonnen, Adane Mihret, Muluwork Getahun, Tsegaye Hailu, Sabeen Sidiki, Holden V. Kelley, Julia M. Scordo, W. Garrett Hunt, Xueliang Pan, Joan-Miquel Balada-Llasat, Wondwossen Gebreyes, Carlton A. Evans,Abraham Assefa, Jordi B. Torrelles, Shu-Hua Wang, Tamrat Abebe. Evaluation of the TB Culture Color Plate Test for Rapid Detection of Drug susceptible and Drug-resistant Mycobacterium tuberculosis in a resource-limited Setting, Addis Ababa, Ethiopia PLOS One in press 2019, accepted 20190412