SUMMARY
The MDR/XDR-TB Colour Test is a simple, safe, low-cost, appropriate-technology test for diagnosing TB and concurrently determining antibiotic-susceptibility testing. This test helps ensure that patients in resource-constrained settings receive the best treatment for their TB disease. The MDR/XDR-TB Colour Test was invented and developed by the IFHAD research group in Peru and has since been evaluated in multiple countries – please see the publications below.
PROTOCOL
The current MDR/XDR-TB Colour Test protocol may be downloaded from this link: https://www.dropbox.com/sh/hircpcbcs2cv6c3/AADeTbxlyH6pX8cF1TLMR1LRa?dl=0
This file includes links to previous versions of the protocol.
Further information is available from carlton.evans@ifhad.org
PUBLICATIONS
B Herrera, R H Gilman, L Grandjean, L Martin, J Alvarado, W Quino, T Valencia, G Sandhu, R Montoya, J Alva, J Franco, M Haro, R Sosa, E Valera,B Valiente, M Rivero, S Carrera, A R Escombe, A Curatola, C A Evans.
Optimisation of TB field testing: In transit sputum decontamination & culture on colorimetric selective media for TB diagnosis & drug-susceptibility testing.
Presentation at the Medecins Sans Frontieres (MSF) symposium on TB field Diagnostics ‘Dying for a test’. 7/11/2007 Capetown. http://www.msfaccess.org/fileadmin/user_upload/diseases/tuberculosis/DX_Abstacts_CT.pdf
S Shell, E Ramos, B Herrera, RH Gilman, W Quino, J Alvarado, T Valencia, G Sandhu, M Rivero, S Carrera, R Montoya, J Alva, J Franco, M Haro, R Sosa, E Valera, B Valiente, A Curatola, AR Escombe, D Moore, P Sheen, C Evans. Enhancing TB culture diagnosis with the colorimetric indicator STC American Society of Tropical Medicine & Hygiene conference 4/11/2007 Philadelphia.
OC Boney, K Zevallos1, RH Gilman, L Caviedes, T Valencia, CO Vidal, AR Escombe, CA Evans.
Evaluation of MODS vs. clinical assessment, sputum microscopy, culture and PCR for diagnosing patients with TB.
Presentation at the Medecins Sans Frontieres (MSF) symposium on TB field Diagnostics ‘Dying for a test’. 7/11/2007 Capetown. http://www.msfaccess.org/fileadmin/user_upload/diseases/tuberculosis/DX_Abstacts_CT.pdf
B Herrera, E Ramos, R Gilman, L Grandjean, L Martin, J Alvarado, W Quino, G Sandhu, J Alva, R Sosa, S Carrera, D Coleman, D Mitchison, C Evans.
A simple colour test for diagnosing MDRTB & XDRTB under field conditions.
CDC symposium, World Conference of the International Union Against TB & Lung Disease Paris, 20/10/2008.
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B Herrera, E Ramos , R Gilman, L Grandjean, L Martin, J Alvarado, T Valencia, W Quino, G Sandhu, J Alva, R Sosa, S Carrera, D Coleman, D Mitchison, C Evans.
Simplifying culture-based TB/MDRTB testing for Peripherals laboratories.
FIND Forum on Recent Advances in TB Diagnostics, Paris 17/10/2008. Abstract published in conference proceedings.
MA Tovar, T Valencia, RH Gilman, L Caviedes, E Ramos, J Alvarado, W Quino, B Herrera, CA Evans.
Validation of the MODS technique for drug-susceptibility testing during TB therapy.
American J Tropical Medicine & Hygiene 2009: 81(5); 212: page 212. Abstract#1317, Presentation Number: 734 Session 125 http://www.ajtmh.org/content/81/5_Suppl_1/201.full.pdf
MA Tovar, T Valencia, J Alvarado, C Rojas, L Caviedes, S Schumacher, R Gilman, C Evans
Rapid direct MDRTB testing better predicts clinical outcome than conventional delayed testing
International Journal of TB and Lung Disease 2010:14(11);S73.-74. http://www.theunion.org/what-we-do/journals/ijtld/body/ABSTRACT_BOOK_2010_Web.pdf
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SG Schumacher, T Valencia, R Montoya, M Tovar, A Gavino, S Carrera, RH Gilman, C Evans
Operational effectiveness of TB culture and drug-susceptibility testing in a high-prevalence setting.
International Journal of Tuberculosis and Lung Disease 2010:14(11);S72.http://www.theunion.org/what-we-do/journals/ijtld/body/ABSTRACT_BOOK_2010_Web.pdf
MA Tovar, C Osorio, E Ramos, M Llacza, B Herrera, SG Schumacher, RH Gilman, C EvansA simple colour test for diagnosing MDRTB & XDRTB under field conditions International Journal of TB and Lung Disease 2010:14(11);S73. http://www.theunion.org/what-we-do/journals/ijtld/body/ABSTRACT_BOOK_2010_Web.pdf
MA Tovar, SG Schumacher, C Osorio, E Ramos, M Llacza, T Valencia, B Herrera, CA EvansComparison of TB diagnosis with the MDR/XDRTB Colour Test and MODS International Journal of TB & Lung Disease 2011:15(11);S274. http://www.worldlunghealth.org/confLille/images/stories/AbstractBook2011_Web.pdf
Rocha C, Tilley H, Ching M, Ramos E, Tovar MT, Rivera E, Cortegana L, Evans CA.
Field assessment of TB culture techniques in a resource-poor jungle setting.
International Journal of TB & Lung Disease 2012:16(12);S430.http://www.worldlunghealth.org/conf2012/images/banners/RANDOMS/ABSTRACT_BOOK_2012_WEB.pdf
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K Toit, S Mitchell, Y Balabanova, CA Evans, T Kummik, V Nikolayevskyy, F Drobniewski
The Colour Test for drug susceptibility testing of TB strains by culture on thin-layer agar
International Journal of TB & Lung Disease 2012 Aug;16(8):1113-8. doi: 10.5588/ijtld.11.0609. http://www.ncbi.nlm.nih.gov/pubmed/22762424
ES Ramos, CE Osorio, TR Valencia, MF Llacza, MA Tovar, R Montoya, T Wingfield, CA Evans
The specificity of the MDR/XDR-TB Colour Test for differentiating Mycobacterium TB from atypical mycobacteria
http://www.bc.lung.ca/association_and_services/documents/2013TheUnion-NARPreliminaryProgram-FINALPROGRAM.pdf page 71
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J Wendell, B Hanson Herrera, E Ramos, MA Tovar, S Schumacher, T Valencia, D Coleman, CA Evans.
The MDR/XDR-TB Colour Test simultaneous culture and drug susceptibility results predict TB outcomes.
International Journal of TB & Lung Disease 2013: 17(12);S271. http://www.worldlunghealth.org/conf2013/images/1_Paris2013/Forms/ABSTRACT_BOOK_2013_Web.pdf
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ES Ramos, D Coleman, CE Osorio, TR Valencia, MF Llacza, MA Tovar, T Wingfield, CA Evans
Carbendazim reduces contamination of TB cultures in the thin layer agar MDR/XDR-TB Colour Test
International Journal of TB & Lung Disease 2013:17(12);S272. http://www.worldlunghealth.org/conf2013/images/1_Paris2013/Forms/ABSTRACT_BOOK_2013_Web.pdf
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Rocha C, Tilley DH., Ching M., Ramos E., Tovar MA., Rivera E., Cortegana L., Evans CA.
Comparison of time to MDR-TB diagnosis with the MDR/XDR-TB Colour Test & current algorithm in a resource-poor jungle setting in Peru
International Journal of TB & Lung Disease 2013:17(12);S472. http://www.worldlunghealth.org/conf2013/images/1_Paris2013/Forms/ABSTRACT_BOOK_2013_Web.pdf
Eric Steve Ramos, Carmen Osorio, Teresa Valencia, Manuel Llacza, Moises Ching, Marco Tovar, Alexander Sloutsky, Carlton A Evans
High TB diagnostic yield from culturing salivary sputum samples
International Journal of TB & Lung Disease 2014:18(11);S285 http://www.theunion.org/what-we-do/journals/ijtld/body/Abstract_Book_2014-Web.pdf
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Marco A Tovar, Teresa Valencia, Silvia Carrera, Eric Ramos, Tom Wingfield, Rosario Montoya, Maribel Rivero, Carlton A Evans
Undiagnosed tuberculosis cases among previously TB-affected families: results of a prevalence survey in Peru
International Journal of TB & Lung Disease 2014:18(11);S221 http://www.theunion.org/what-we-do/journals/ijtld/body/Abstract_Book_2014-Web.pdf
Marco A. Tovar, Eric Ramos, Teresa Valencia, Sumona Datta, Rosario Montoya, Adriana Valencia, Tom Wingfield, Carlton A. Evans
Comparison of the MDR/XDRTB Colour Test, Xpert MTB/Rif and MODS assays to diagnose rifampicin resistant TB
International Journal of TB & Lung Disease 2015:19(12);S368 http://www.theunion.org/what-we-do/journals/ijtld/body/Abstract_Book_2015-Web.pdf
Marco A. Tovar, Tom Wingfield, Matthew J. Saunders, Rosario Montoya, Eric Ramos, Teresa Valencia, Sumona Datta, Carlton A. Evans
Risk factors associated with undetected pulmonary TB in a prevalence survey, Callao, Perú
International Journal of TB & Lung Disease 2016:20(11);S255
http://www.theunion.org/what-we-do/journals/ijtld/body/Abstract_Book_2016-Web.pdf
Sumona Datta, Marco A Tovar, Teresa R Valencia, Eric S Ramos, Rosario Montoya, James Lewis, Carlton A. Evans
Sputum collection factors affecting diagnostic yield
International Journal of TB & Lung Disease 2017:21(11); S188 http://www.abstractserver.com/TheUnion2017/TheUnion2017_Abstracts_Web.pdf
Annie Zhang, Enoch Jumbe, Robert Krysiak, Sabeen Sidiki, Holden V. Kelley, Elly K. Chemey, Chancy Kamba, Victor Mwapasa, Juan I. Garcà a, Alison Norris, Xueliang J. Pan, Carlton Evans, Shu-Hua Wang, Jesse J. Kwiek, Jordi B. Torrelles
Low-cost diagnostic test for susceptible and drug-resistant tuberculosis in rural Malawi
African Journal of Laboratory Medicine 2018 Vol 7(1); a690 DOI:
Open access https://doi.org/10.4102/ajlm.v7i1.690
Sumona Datta, Marco A Tovar, Thomas Wingfield, Rosa Patrocinio, Rosario Montoya, Teresa Valencia, Robert H Gilman,Carlton A Evans.
Monitoring TB treatment response with conventional laboratory tests during the first 14-days of therapy has no clinical value.
European Conference clinical infectious diseases and microbiology, in press. Poster presentation, 24th April 2018.
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S Datta, D Huff, L Quevedo Cruz, J Quispe Cotache, R Montoya, MA Tovar, M Saunders, JJ Lewis, RH Gilman, CA Evans. A quantitative assessment of general wellbeing in TB-affected people
Oral abstract session OA20-328-27, Saturday, 27th  October.
International Journal of TB & Lung Disease 2018:22(11); S434. https://www.abstractserver.com/TheUnion2018/TheUnion2018_Abstracts_Web.pdf
A. Shibabaw, B. Gelaw, H. Kelley, J. Balada-Llasat, C. Evans, S. Wang, J. Torrelles, B. Tessema.
Accuracy of the color plate micro-colony detection for the diagnosis of Mycobacterium TB complex in NW Ethiopia.
Tuberculosis. 2019; 114:54-60.
Biruk Mekonnen, Adane Mihret, Muluwork Getahun, Tsegaye Hailu, Sabeen Sidiki, Holden V. Kelley, Julia M. Scordo, W. Garrett Hunt, Xueliang Pan, Joan-Miquel Balada-Llasat, Wondwossen Gebreyes, Carlton A. Evans,Abraham Assefa, Jordi B. Torrelles, Shu-Hua Wang, Tamrat Abebe. Evaluation of the TB Culture Color Plate Test for Rapid Detection of Drug susceptible and Drug-resistant Mycobacterium tuberculosis in a resource-limited Setting, Addis Ababa, Ethiopia PLOS One in press 2019, accepted 20190412